Journal: Oncology Reports

Article Title: NONHSAG028908.3 sponges miR‑34a‑5p to promote growth of colorectal cancer via targeting ALDOA

doi: 10.3892/or.2023.8526

Figure Lengend Snippet: Expression level of NONHSAG028908.3 in CRC tissues and cells. (A) Data obtained from TCGA database revealing the expression of NONHSAG028908.3 in CRC tissues and normal tissues. (B) The relative expression levels of NONHSAG028908.3 in four CRC cell lines were detected via RT-qPCR. (C) LoVo or (D) HCT116 cells were transfected with si-NC, si-NONHSAG028908.3-1 or si-NONHSAG028908.3-2, respectively. RT-qPCR was used to evaluate the transfection efficiency. Th results are presented as the mean ± SD. **P<0.01 and ***P<0.001. CRC, colorectal cancer; TGCA, The Cancer Genome Atlas; RT-qPCR, reverse transcription-quantitative PCR; FPKM, fragments per kilobase of exon model per million mapped fragments; si-, siRNA; NC, negative control.

Article Snippet: The normal human colon mucosal epithelial cell line (NCM460; cat. no. CP-H040) and human CRC cell lines (LoVo, cat. no. CL-0144; HCT116, cat. no. CL-0096; and Caco-2, cat. no. CL-0050) were purchased from Procell Life Science & Technology Co., Ltd. HT-29 (cat. no. ZQ0057), a CRC cell line, was obtained from Shanghai Zhong Qiao Xin Zhou Biotechnology Co., Ltd.

Techniques: Expressing, Quantitative RT-PCR, Transfection, Reverse Transcription, Real-time Polymerase Chain Reaction, Negative Control

Journal: Oncology Reports

Article Title: NONHSAG028908.3 sponges miR‑34a‑5p to promote growth of colorectal cancer via targeting ALDOA

doi: 10.3892/or.2023.8526

Figure Lengend Snippet: Silencing of NONHSAG028908.3 suppresses the cell growth of CRC cells. The cell growth of transfected LoVo and HCT116 cells was examined by (A and B) MTT assay and (C and D) BrdU staining. Scale bar, 50 µm. The results are presented as the mean ± SD. **P<0.01 and ***P<0.001. CRC, colorectal cancer; si-, siRNA; NC, negative control.

Article Snippet: The normal human colon mucosal epithelial cell line (NCM460; cat. no. CP-H040) and human CRC cell lines (LoVo, cat. no. CL-0144; HCT116, cat. no. CL-0096; and Caco-2, cat. no. CL-0050) were purchased from Procell Life Science & Technology Co., Ltd. HT-29 (cat. no. ZQ0057), a CRC cell line, was obtained from Shanghai Zhong Qiao Xin Zhou Biotechnology Co., Ltd.

Techniques: Transfection, MTT Assay, BrdU Staining, Negative Control

Journal: Oncology Reports

Article Title: NONHSAG028908.3 sponges miR‑34a‑5p to promote growth of colorectal cancer via targeting ALDOA

doi: 10.3892/or.2023.8526

Figure Lengend Snippet: Knockdown of NONHSAG028908.3 restrains CRC cell migration and invasion. (A) A wound healing assay was performed to determine cell migration. Scale bar, 200 µm. (B) A Transwell assay was used to determine cell invasion. Scale bar, 100 µm. (C) The expression levels of MMP-2 and MMP-9 were assessed by western blot assay. The results are presented as the mean ± SD. **P<0.01 and ***P<0.001. CRC, colorectal cancer; MMP, matrix metalloproteinase; si-, siRNA; NC, negative control.

Article Snippet: The normal human colon mucosal epithelial cell line (NCM460; cat. no. CP-H040) and human CRC cell lines (LoVo, cat. no. CL-0144; HCT116, cat. no. CL-0096; and Caco-2, cat. no. CL-0050) were purchased from Procell Life Science & Technology Co., Ltd. HT-29 (cat. no. ZQ0057), a CRC cell line, was obtained from Shanghai Zhong Qiao Xin Zhou Biotechnology Co., Ltd.

Techniques: Knockdown, Migration, Wound Healing Assay, Transwell Assay, Expressing, Western Blot, Negative Control

Journal: Oncology Reports

Article Title: NONHSAG028908.3 sponges miR‑34a‑5p to promote growth of colorectal cancer via targeting ALDOA

doi: 10.3892/or.2023.8526

Figure Lengend Snippet: Knockdown of ALDOA inhibits CRC cell proliferation and migration. (A and B) Both LoVo and HCT116 cells were transfected with si-ALDOA, and the expression efficiency of ALDOA was assessed via RT-qPCR. (C and D) Cell proliferation was evaluated using MTT assay. (E and F) The cell migration rate of LoVo and HCT116 cells was analyzed. (G and H) The representative images of cell migration were captured. Scale bar, 200 µm. The results are presented as the mean ± SD. **P<0.01 and ***P<0.001. ALDOA, aldolase, fructose-bisphosphate A; CRC, colorectal cancer; si-, siRNA; RT-qPCR, reverse transcription-quantitative PCR; NC, negative control.

Article Snippet: The normal human colon mucosal epithelial cell line (NCM460; cat. no. CP-H040) and human CRC cell lines (LoVo, cat. no. CL-0144; HCT116, cat. no. CL-0096; and Caco-2, cat. no. CL-0050) were purchased from Procell Life Science & Technology Co., Ltd. HT-29 (cat. no. ZQ0057), a CRC cell line, was obtained from Shanghai Zhong Qiao Xin Zhou Biotechnology Co., Ltd.

Techniques: Knockdown, Migration, Transfection, Expressing, Quantitative RT-PCR, MTT Assay, Reverse Transcription, Real-time Polymerase Chain Reaction, Negative Control

Journal: Frontiers in Physiology

Article Title: Ascorbic Acid Chemosensitizes Colorectal Cancer Cells and Synergistically Inhibits Tumor Growth

doi: 10.3389/fphys.2018.00911

Figure Lengend Snippet: Concentrations of ascorbic acid (AA), 5-Fluorouracil (5-FU), oxaliplatin (Oxa), and irinotecan (Iri) present in each mixture, for f = 0.75, to evaluate cell proliferation of C2BBe1, LS1034, and WiDr cell lines.

Article Snippet: Two human cell lines of CRC, C2BBe1 [clone of Caco-2] (ATCC Cat# CRL-2102, RRID:CVCL_1096 ) and WiDr (ATCC Cat# CCL-218, RRID:CVCL_2760 ), were cultured in Dulbecco’s Modified Eagle’s Medium, DMEM (Sigma) and the another one, LS1034 (ATCC Cat# CRL-2158, RRID:CVCL_1382 ), was cultured in Roswell Park Memorial Institute Medium, RPMI-1640 (Sigma).

Techniques:

Journal: Frontiers in Physiology

Article Title: Ascorbic Acid Chemosensitizes Colorectal Cancer Cells and Synergistically Inhibits Tumor Growth

doi: 10.3389/fphys.2018.00911

Figure Lengend Snippet: Concentrations of AA, 5-FU, Oxa, and Iri used in combined therapy (CT) for C2BBe1, LS1034 and WiDr cell lines for flow cytometry and western blot studies.

Article Snippet: Two human cell lines of CRC, C2BBe1 [clone of Caco-2] (ATCC Cat# CRL-2102, RRID:CVCL_1096 ) and WiDr (ATCC Cat# CCL-218, RRID:CVCL_2760 ), were cultured in Dulbecco’s Modified Eagle’s Medium, DMEM (Sigma) and the another one, LS1034 (ATCC Cat# CRL-2158, RRID:CVCL_1382 ), was cultured in Roswell Park Memorial Institute Medium, RPMI-1640 (Sigma).

Techniques: Flow Cytometry, Western Blot

Journal: Frontiers in Physiology

Article Title: Ascorbic Acid Chemosensitizes Colorectal Cancer Cells and Synergistically Inhibits Tumor Growth

doi: 10.3389/fphys.2018.00911

Figure Lengend Snippet: IC 50 values (in μM) of 5-FU, Oxa, and Iri, obtained in monotherapy (MT) and in combined therapy with AA (CT), with f = 0.5 or f = 0.75, obtained after treatment of C2BBe1, LS1034, and WiDr cells for 24, 48, 72, and 96 h.

Article Snippet: Two human cell lines of CRC, C2BBe1 [clone of Caco-2] (ATCC Cat# CRL-2102, RRID:CVCL_1096 ) and WiDr (ATCC Cat# CCL-218, RRID:CVCL_2760 ), were cultured in Dulbecco’s Modified Eagle’s Medium, DMEM (Sigma) and the another one, LS1034 (ATCC Cat# CRL-2158, RRID:CVCL_1382 ), was cultured in Roswell Park Memorial Institute Medium, RPMI-1640 (Sigma).

Techniques:

Journal: Frontiers in Physiology

Article Title: Ascorbic Acid Chemosensitizes Colorectal Cancer Cells and Synergistically Inhibits Tumor Growth

doi: 10.3389/fphys.2018.00911

Figure Lengend Snippet: Combination index (CI) values obtained for C2BBe1, LS1034 and WiDr cells, for f = 0.5 and f = 0.75, and for each incubation time.

Article Snippet: Two human cell lines of CRC, C2BBe1 [clone of Caco-2] (ATCC Cat# CRL-2102, RRID:CVCL_1096 ) and WiDr (ATCC Cat# CCL-218, RRID:CVCL_2760 ), were cultured in Dulbecco’s Modified Eagle’s Medium, DMEM (Sigma) and the another one, LS1034 (ATCC Cat# CRL-2158, RRID:CVCL_1382 ), was cultured in Roswell Park Memorial Institute Medium, RPMI-1640 (Sigma).

Techniques: Incubation

Journal: Frontiers in Physiology

Article Title: Ascorbic Acid Chemosensitizes Colorectal Cancer Cells and Synergistically Inhibits Tumor Growth

doi: 10.3389/fphys.2018.00911

Figure Lengend Snippet: Analysis of cell viability and types of cell death induced in C2BBe1, LS1034, and WiDr cells after exposure to AA and 5-FU, oxaliplatin (Oxa) or irinotecan (Iri) alone or in combined therapy (CT) for 48 h. The results are represented in percentage (%) of viable cells (V), initial apoptosis (IA), late apoptosis/necrosis (LA/N) and necrosis (N). The results express the mean and standard error of at least three independent experiments, in duplicate. Statistically significant differences relative to control are marked with ∗ p < 0.05, ∗∗ p < 0.01 and ∗∗∗ p < 0.001.

Article Snippet: Two human cell lines of CRC, C2BBe1 [clone of Caco-2] (ATCC Cat# CRL-2102, RRID:CVCL_1096 ) and WiDr (ATCC Cat# CCL-218, RRID:CVCL_2760 ), were cultured in Dulbecco’s Modified Eagle’s Medium, DMEM (Sigma) and the another one, LS1034 (ATCC Cat# CRL-2158, RRID:CVCL_1382 ), was cultured in Roswell Park Memorial Institute Medium, RPMI-1640 (Sigma).

Techniques: Control

Journal: Frontiers in Physiology

Article Title: Ascorbic Acid Chemosensitizes Colorectal Cancer Cells and Synergistically Inhibits Tumor Growth

doi: 10.3389/fphys.2018.00911

Figure Lengend Snippet: Analysis of cell cycle alterations in C2BBe1, LS1034 and WiDr cells after exposure to AA and 5-FU, Oxa or Iri alone or in CT for 48 h. The results are represented in percentage (%) of cells in the pre-G0/G1 peak and in the G0/G1, S and G2/M phases of the cell cycle. The results express the mean and standard error of at least three independent experiments, in duplicate. Statistically significant differences are marked with ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001.

Article Snippet: Two human cell lines of CRC, C2BBe1 [clone of Caco-2] (ATCC Cat# CRL-2102, RRID:CVCL_1096 ) and WiDr (ATCC Cat# CCL-218, RRID:CVCL_2760 ), were cultured in Dulbecco’s Modified Eagle’s Medium, DMEM (Sigma) and the another one, LS1034 (ATCC Cat# CRL-2158, RRID:CVCL_1382 ), was cultured in Roswell Park Memorial Institute Medium, RPMI-1640 (Sigma).

Techniques:

Journal: Frontiers in Physiology

Article Title: Ascorbic Acid Chemosensitizes Colorectal Cancer Cells and Synergistically Inhibits Tumor Growth

doi: 10.3389/fphys.2018.00911

Figure Lengend Snippet: Analysis of BAX and BCL-2 expression and mitochondrial membrane potential (Δψ m ) after exposure of C2BBe1, LS1034 and WiDr cells to AA and Oxa alone or in CT for 48 h. (A) Mitochondrial membrane potential (Δψ m ) values are expressed as aggregates/monomers (A/M) and (B) apoptosis-related proteins are expressed as BAX/BCL-2 ratio for each condition and cell line. The decrease in the A/M ratio is directly correlated with decreased Δψ m . All results express the mean and standard deviation of at least four independent experiments, in duplicate. Statistically significant differences are marked with ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001.

Article Snippet: Two human cell lines of CRC, C2BBe1 [clone of Caco-2] (ATCC Cat# CRL-2102, RRID:CVCL_1096 ) and WiDr (ATCC Cat# CCL-218, RRID:CVCL_2760 ), were cultured in Dulbecco’s Modified Eagle’s Medium, DMEM (Sigma) and the another one, LS1034 (ATCC Cat# CRL-2158, RRID:CVCL_1382 ), was cultured in Roswell Park Memorial Institute Medium, RPMI-1640 (Sigma).

Techniques: Expressing, Membrane, Standard Deviation

Journal: Frontiers in Physiology

Article Title: Ascorbic Acid Chemosensitizes Colorectal Cancer Cells and Synergistically Inhibits Tumor Growth

doi: 10.3389/fphys.2018.00911

Figure Lengend Snippet: Analysis of caspase-9 expression after exposure of C2BBe1, LS1034 and WiDr cells to AA and Oxa alone or in CT for 48 h. (A) The plot represents the expression of caspase-9 (46 kDa) and a 35 kDa cleavage product. The results are expressed as the ratio between the fluorescence intensity of caspase-9 and β-actin, normalized to the control. All results express the mean and standard error of at least six independent experiments. Statistically significant differences are marked with ∗ p < 0.05 and ∗∗ p < 0.01. (B) Representative immunoblot of caspase-9 expression.

Article Snippet: Two human cell lines of CRC, C2BBe1 [clone of Caco-2] (ATCC Cat# CRL-2102, RRID:CVCL_1096 ) and WiDr (ATCC Cat# CCL-218, RRID:CVCL_2760 ), were cultured in Dulbecco’s Modified Eagle’s Medium, DMEM (Sigma) and the another one, LS1034 (ATCC Cat# CRL-2158, RRID:CVCL_1382 ), was cultured in Roswell Park Memorial Institute Medium, RPMI-1640 (Sigma).

Techniques: Expressing, Fluorescence, Control, Western Blot